Coding

Part:BBa_K784031:Design

Designed by: Noa Katz   Group: iGEM12_Technion   (2012-09-17)


xhoI - xyIE - xmaI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 346
    Illegal NgoMIV site found at 518
    Illegal AgeI site found at 869
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to use retriction enzymes that don't appear in the xyIE gene.


Source

The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh. The part's name: BBa_J33204

References